Sarscov2 detection in formalinfixed paraffinembedded. The reliaprep blood gdna miniprep system provides a complete, readytouse method for purification of gdna from up to 200. Maxwell rsc 48 instrument operating manual promega. Maxwell rsc tissue dna kit technical manualpdf 636 kb english. Maxwell rsc instrument operating manual, tm411 promega. The maxwell rsc instrument is for research use only. Maxwell rsc mirna tissue kit protocol promega corporation. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Description the maxwell rsc ffpe plus dna kita is used with maxwell instruments to provide a simple method for efficient, automated purification of dna gdna from ffpe formalinfixed, paraffin. Technical manual maxwell rsc buffy coat dna kit promega. Maxwell rsc whole blood dna cartridge preparation continued 3.
Maxwell rsc 48 instrument operating manualpdf 12629 kb english. Technical manual maxwell rsc cell dna purification kit instructions for use of product as70 note. This dna purification chapter addresses general information on the basics of dna isolation, plasmid growth and dna quantitation as well as how purification by silica can help increase your productivity so you spend less time purifying dna and. The maxwell rsc instrument is controlled by a graphical user interface running on a tablet pc. Cross contamination is minimized and the instrument is supported by the promega technical support and service you have come to trust over the past 35 years. Dna can be extracted from up to 16 preprocessed samples in less than 60 minutes and can be used directly in a variety of downstream applications, including pcr. Up to 16 samples can be prepared simultaneously in 2560 minutes, depending on sample type. Maxwell rsc instrument operating manual promega corporation. Using prefilled cartridges and preprogrammed methods, the maxwell rsc offers consistent, reliable dna or rna extraction in 2560 minutes, depending upon sample type. To use the maxwell rsc cell dna purificationkit, you must have the cell dna method loaded on the maxwell rsc or maxwell rsc 48 instrument. When a user loads portal access from another pc, the username is shared with portal. The maxwell rapid sample concentrator 48 rsc 48 instrument provides automated nucleic acid purification for a range of research sample types. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. The maxwell rsc instrument is a compact, automated nucleic acid purification platform that processes up to 16 samples simultaneously.
We can also develop a customized protocol based on existing kits to meet your specific requirements. The reliaprep gdna tissue miniprep system provides a complete, readytouse method for purification of gdna from up to 25mg of tissue, a buccal swab, or a 1cm mouse tail snip, obtaining intact gdna without the use of ethanol washes or precipitations. Email promega technical services if you have questions on use of this system. Maxwell rsc simplyrna blood kit technical manualpdf. The maxwell rsc dna ffpe kit is used with the maxwell rsc instrument to purify rna from formalinfixed, paraffinembedded ffpe mammalian tissue samples. Contact your local promega representative for the most up to date information. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Guidelines related to safety procedures in the laboratory have been released. The purification methods use sample lysis and binding to paramagnetic particles as the primary separation principle.
The instrument is controlled through a graphical user interface running on a tablet pc. Promega manufacturing and delivery systems continue to be fully operational during. This quick protocol provides instructions for use of the maxwell rsc tissue dna kit with the maxwell rsc. Operating instructions for the maxwell rsc 48 instrument. Occasionally, an existing protocol method may have to be updated. The maxwell rsc 48 instrument is designed for use by laboratory professionals. The maxwell rapid sample concentrator rsc instrument is a platform for automated purification of nucleic acid from a range of sample types. Not all sample preprocessing methods require or support all of the listed parameters. Using this kit and the maxwell rsc instrument, mirna can be purified from 1 16 samples in about 90 minutes. If a larger volume of eluate is desired, the purified nucleic acid can be eluted in up to 150l of. In the severe acute respiratory syndrome coronavirus 2 sarscov2 pandemic, pathologists can be exposed to infection handling surgical specimens. The maxwell rsc dna or rna extraction methods start with cartridges prefilled. Maxwellr 16 instrument operating manual tm274 promega.
Checking this box will create a pdf version of exported results in. Added new product component lytic enhancer and updated protocol instructions for its use sections 4. Developmental validation of the maxwell fsc dna iq. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll. The maxwell rapid sample concentrator rsc instrument provides automated nucleic acid purification for a range of research sample types. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Maxwell rsc cell dna purification kit technical manual, tm418. The maxwell rsc tissue dna kit is a simple, automated method for extraction of. Maxwell rsc instrument operating manualpdf 6290 kb english. Maxwellr 16 mdx instrument technical manual, tm320. Description the maxwell rsc dna ffpe kita is used with the maxwell rsc or maxwell rsc 48 instrument to provide a simple method for efficient, automated purification of dna gdna from ffpe formalin. However, there is a lack of studies performed on biopsy and surgical resection specimens. The maxwell rsc instrument is designed for use by laboratory professionals.
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